Page 2 Angiogenesis The process of new blood vessel formation   Assays GlycoTech employs four assays to investigate angiogenesis. These are: (1) Endothelial cells on matrigel, (2) Endothelial cell chemotaxis, (3) Umbilical artery outgrowth, and (4) Chick chorioallantoic membrane development. Endothelial Cells on Matrigel Human umbilical vein endothelial cells (huvecs) are isolated from fresh umbilical cords and used at low passage number in the assay. Huvecs are seeded on a surface coated with matrigel. The huvec cells migrate quickly and organize into a network of pre-tubules in the first 2-3 hours. A typical image of the network is seen below and is qunatified by the pixel count of the processed image.