CD32(8.7) , Biotin conjugate , 0.1mg / mL

This MAb reacts with a CD32 (FcgRII) epitope distinct from that defined by MAb 8.26 and the epitope overlaps with that of MAb 7.30 (cluster 4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis , cytotoxicity , and immunomodulation as well as platelet aggregation. Three genes (A , B , and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing , i.e. , IIa1 , IIa2 , IIb1 , IIb2 , IIb3 and IIc. Monocytes/macrophages , placental trophoblasts and endothelial cells express all isoforms. In addition , the IIb isoform is expressed by B cells , and the IIa isoform by platelets , granulocytes and , weakly , by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.Primary antibodies are available purified , or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets , because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.