CD16(ICO-116) , Biotin conjugate , 0.1mg / mL

This antibody recognizes CD16 (FcγRIII) , the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16 , CD16A (FcγRIIIA) , which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcγRIIIB) , which exist as a monomeric GPI-anchored form in neutrophils. Furthermore , there are two known polymorphisms of CD16B , NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding , both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC) , phagocytosis , degranulation and proliferation.Primary antibodies are available purified , or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets , because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.