Involucrin (Squamous Cell Terminal Differentiation Marker)(rIVRN/827) , Biotin conjugate , 0.1mg / mL

Recognizes a protein of 66 kDa-170 kDa , identified as involucrin. In Western blotting of cultured human keratinocytes , this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells , a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey , 66 kDa in dog , and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia , including the cornea , which lacks a distinct cornified layer. In normal epidermis , it is first expressed in the upper spinous layers , and in keratinocyte cultures , all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias , involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions , and is reduced in severe dysplasias of the larynx and cervix. Primary antibodies are available purified , or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets , because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.