HIF1 alpha (Hypoxia-Inducible Factor 1-alpha)(ESEE122) , 1mg / mL

HIF1 (hypoxia-inducible factor 1) , a heterodimeric transcription factor complex central to cellular response to hypoxia , consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per , ARNT , Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway) , growth factors , oncogenic activation , or loss of tumor suppressor function etc. In normoxic cells , HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine , cobalt etc. ) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells , whereas , contrastingly , HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response , HIF1s regulates the transcription of genes responsible for angiogenesis , erythropoiesis/iron-metabolism , glucose metabolism , cell proliferation/survival , adipogenesis , carotid body formation , B lymphocyte development and immune reactions. Primary antibodies are available purified , or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets , because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.