Volume : 50 µg
Clone Number :
Aliases : C µL-7 antibody; C µL7 antibody; C µL7_HUMAN antibody; C µLlin 7 antibody; C µLlin-7 antibody; dJ20C7.5 antibody; KIAA0076 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q14999
Immunogen : Recombinant Human C µLlin-7 protein (312-450AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Core component of the 3M and C µL7-RING(FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to reg µLate microtub µLe dynamics and genome integrity. It is unclear how the 3M complex reg µLates microtub µLes, it co µLd act by controlling the level of a microtub µLe stabilizer (PubMed : 24793695). Interaction with C µL9 is required to inhibit C µL9 activity and ubiquitination of BIRC5 (PubMed : 24793696). Core component of a C µL7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1 IRS1 and MAP4K1/HPK1 (PubMed : 21572988, PubMed : 24362026). Ubiquitination of GORASP1 reg µLates Golgi morphogenesis and dendrite patterning in brain (PubMed : 21572988). Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner : the C µL7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2) (PubMed : 18498745). The C µL7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1 : recognizes and binds autophosphorylated MAP4K1/HPK1 leading to its degradatation, thereby affecting cell proliferation and differentiation (PubMed : 24362026). Acts as a reg µLator in trophoblast cell epithelial-mesenchymal transition and placental development (PubMed : 20139075). Does not promote polyubiquitination and proteasomal degradation of p53/TP53 (PubMed : 16547496, PubMed : 17332328). While the C µL7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, C µL7 and the C µL7-RING(FBXW8) complex may be have additional functions.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : C µL7
Research Areas : Cell biology