Creating native carbohydrate - protein interactions to advance human health
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01 | Swiss Institute of Bioinformatics | www.sib.swiss
02 | Department of Structure and Function of Saccharides, Slovak Academy of Science | www.modlab.chem.sk
03 | Centre for cooperative research in biomaterials | www.glycotechnology.net
04 | CNRS-Cermav | www.cermav.cnrs.fr
05 | ETH Zurich, Institute of Microbiology | https://micro.biol.ethz.ch/
06 | Oxford Glycobiology Institute | www.bioch.ox.ac.uk/glycob
07 | Centre for Proteomics & Metabolomics at LUMC | https://cpm.lumc.nl/glycomics-and-glycoproteomics
08 | KTH, Division of Glycoscience | www.kth.se/che/glykovetenskap/division-of-glycoscience-1.785898
09 | VIB-UGent Center for Medical Biotechnology | www.vib.be/labs/nico-callewaert-lab
10 | Laboratory of Fungal Glycobiology, Polish Academy of Sciences
www.ibb.waw.pl/en/structure/ibb-departments/laboratory-fungal-glycobiology
11 | Centre for Chemistry and Biochemistry, Universidade de Lisboa | http://cqb.fc.ul.pt/research/carbohydrate-chemistry
12 | Max Planck Institute of Colloids and Interfaces | www.mpikg.mpg.de/en/bs
13 | Centre for Glycobiology, University of Liverpool | www.liverpool.ac.uk/integrative-biology/staff/jeremy-turnbull
14 | Carbohydrate Competence Centre | www.cccresearch.nl
15 | Copenhagen Center for Glycomics | www.glycomics.ku.dk
16 | Department of Carbohydrates and Cereals | https://sch.vscht.cz/veda-a-vyzkum
17 | Norwegian Biopolymer Laboratory, Norwegian University of Science & Technology
www.ntnu.edu/ibt/research/biopol#/view/about
18 | Department of Organic Chemistry at University of Naples Federico II
https://www.scienzechimiche.unina.it/en_GB/ricerca/la-nostra-ricerca
LIST OF SUPPLIERS FOR GLYCOBIOTECHNOLOGY APPLICATIONS
Carbosynth | www.carbosynth.com
Cyclolab | www.cyclolab.hu
Dextra Laboratories | www.dextrauk.com
Elicityl | www.elicityl-oligotech.com
Glycouniverse | www.glycouniverse.com
Megazyme | www.megazyme.com
Prozomix | www.prozomix.com
Beginner
BIOINFORMATICS DATABASE | GLYCAN AND GLYCOME
Selection of freely accessible databases
► GlyTouCan – International glycan repository: https://glytoucan.org
► GlyConnect – Curated intact glycoproteins: https://glyconnect.expasy.org
► CAZy – Curated families of structurally and catalytically related carbohydrate enzymes: http://www.cazy.org
► GlycoEpitope – Curated carbohydrate antigens and glycan epitopes: https://www.glycoepitope.jp/epitopes
► MatrixDB – Curated matrix protein-protein and protein-polysaccharide interactions: http://matrixdb.univ-lyon1.fr
► UniCarb-DB – Curated glycomics mass spectrometry: https://unicarb-db.expasy.org
► UniLectin – Curated structurally classified lectins: https://www.unilectin.eu
Selection of freely accessible tools
► GlycoMod – Predicts oligosaccharide structures on proteins: https://web.expasy.org/glycomod
► GlycoDomainViewer – Predicts the glycosylated state of proteins: https://glycodomain.glycomics.ku.dk
► O-Glycologue – Simulates and predicts glycan biosynthesis: https://glycologue.org
► GLyCAM – Predicts the 3D structure of carbohydrates and glycoconjugates: http://glycam.org
► SweetUnityMol – Display 3D structures: https://sourceforge.net/projects/unitymol
Selection of freely accessible portals
► Glyco3D – 3D structures (saccharides, carbohydrate recognizing proteins): http://glyco3d.cermav.cnrs.fr/home.php
► Glycomics@ExPASy – Bioinformatics for glycoscience in Europe: https://www.expasy.org/glycomics
► GlyCosmos – Bioinformatics for glycoscience in Asia: https://glycosmos.org
► GlyGen – Bioinformatics for glycoscience in the US: https://glygen.org
Expert
DEEP UNDERSTANDING OF CELL SURFACE GLYCANS
ON MICROBES
Highly targeted and effective carbohydrate-based vaccines can only be developed from a deep understanding
of the molecular structure and conformation of the relevant cell surface polysaccharides on bacteria. This will
also enable faster and accurate diagnosis for patients.
LARGE SCALE PRODUCTION OF POLYSACCHARIDES
Developing robust chemical and enzymatic synthesis
platforms for production of specific oligosaccharide
antigen for vaccine development is a priority. Modification of naturally available sugars and the development
of rapid automated synthesis of target antigens will
accelerate vaccine design and development.
Expert
GLYCONCONJUGATE AND ADJUVANT DESIGN
Carbohydrate-based vaccines require a protein carrier to
establish and trigger long-term protection (especially in infants, children and in the elderly population). The method
to attach sugars to these carriers (also known as conjugation) is often complex (or complicated) and expensive. New
methods should allow full control over the modification of
the protein with batch to batch consistency.
NEW BUSINESS AND FUNDING MODELS
Cost sharing models for SMEs and incentives for large
biopharmaceutical companies are required to facilitate
antibiotic discovery. Require new collaborative framework
between industry and academia (public private partnerships) to mitigate issues surrounding IP ownership. Open
source innovation, collaborative partnership and fast track
approval process will persuade public-private investments