Cyclin B1 (G2- & M-phase Cyclin)(CCNB1/2978R) , Biotin conjugate , 0.1mg / mL

Recognizes a protein of 55-62 kDa , identified as cyclin B1. In mammals , cyclin B associates with inactive p34cdc2 , which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis , when Cdc25 , a specific protein phosphatase , dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So , cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified , or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets , because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.