Cell Adhesion Apparatus
- Parallel Plate Flow Chambers
- Wash Chamber for Static Cell Adhesion
- Chamber for Cell Adhesion on TLC Plates
Parallel Plate Flow Chambers
Human Neutrophils Rolling on Human Endothelial Cells Expressing E-selectin | Parallel plate flow chambers are used in dynamic studies of cell adhesion under well-defined shear forces. Various gasket sizes allow flexibility in experimental design to operate with limited quantities of cells and/or reagents. (Refer to Protocol 7) |
Circular Flow Chamber (for 35mm tissue culture dishes)
31-001 Circular Flow Chamber Kit ($550.00) |
Flow Chamber Kit Includes: Two flow decks 8 silicon rubber gaskets (two of each size) Flow Width = 0.25 cm, Thickness = 0.005 in Flow Width = 0.25 cm, Thickness = 0.010 in Flow Width = 0.50 cm, Thickness = 0.010 in Flow Width = 1.00 cm, Thickness = 0.010 in 24 polypropylene tubing connectors (six of each) Threaded 1/16 in. straight and L-connectors Male and Female taper luer to 1/16 in. connectors
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| Replacement Gaskets and Coverslips for the Circular Flow Chamber | |||
| 31-002 | Four Gaskets (Flow Width = 0.25 cm, Thickness = 0.005 in) | $25.00 | |
| 31-003 | Four Gaskets (Flow Width = 0.25 cm, Thickness = 0.010 in) | $25.00 | |
| 31-004 | Four Gaskets (Flow Width = 0.50 cm, Thickness = 0.010 in) | $25.00 | |
| 31-005 | Four Gaskets (Flow Width = 1.00 cm, Thickness = 0.010 in) | $25.00 | |
| 31-008 | Circular Glass Coverslips (pack of 30, 35 mm, #2) | $25.00 |
Rectangular Flow Chamber (for standard microscope slide)
31-010 Rectangular Flow Chamber Kit $490.00 |
Flow Chamber Kit Includes: One flow deck (cast acrylic) 4 silicon rubber gaskets (two of each size) Flow Width = 1.00 cm, Thickness = 0.005 in Flow Width = 1.00 cm, Thickness = 0.010 in 24 polypropylene tubing connectors (six of each) Threaded 1/16 in. straight and L-connectors Male and Female taper luer to 1/16 in. connectors
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| Replacement Gaskets for the Rectangular Flow Chamber | |||
| 31-011 | Four Gaskets (Flow Width = 1.0 cm, Thickness = 0.005 in) | $25.00 | |
| 31-012 | Four Gaskets (Flow Width = 1.0 cm, Thickness = 0.010 in) | $25.00 |
WASH CHAMBER for Assaying Cell Adhesion under Static Conditions
31-006 Static Cell Adhesion Wash Chamber $290.00 | The use of convenient multiwell plates to assay the adhesion of cells to immobilized molecules under static conditions is usually difficult due to the uncontrolled shear forces normally generated while washing the plate. The Wash Chamber allows the wells of the multiwell plate to be washed by inversion in a liquid-filled environment. Unbound cells are separated from the wells by gravity thereby eliminating shear and the passage of the adherent cells through a liquid/air interface.
Refer to Protocol 8 |
CELL ADHESION CHAMBER for TLC Plates
31-007 Cell Adhesion Chamber for TLC Plates $320.00
| Cell surface carbohydrates act as specific recognition molecules for intercellular adhesion among certain cells. Glycosphingolipids represent a group of cell surface glycoconjugates that are easily separated and analyzed by thin layer chromatography. The cell adhesion chamber was specifically designed to permit the assay of cell adhesion directly on glycosphingolipids separated on thin layer chromatograms. Cells are centrifuged directly on the TLC plate to form immediate close contact with chromatographed glycosphingolipids. The position of the bound cells corresponds to the Rf value of the glycosphingolipid ligand which may be used for purification and structural characterization of the functional carbohydrate sequence. Refer to Protocol 9 |
