Species Reactivity : Human (Homo sapiens)
UniProt : N/A
Abbreviation : Anti-IL6 autoantibody
Alternative Names : N/A
Application : ELISA
Range : 7.8-500 pg/mL
Sensitivity : 3.2 pg/mL
Intra-AssayCV : ?5.1%
Inter-AssayCV : ?8.9%
Recovery : 0.85
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Competitive ELISA
Analysis Method?? : Qualitative
Test principle : This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-IL6 autoantibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conj µgated Anti-IL6 autoantibody and incubated. The competitive inhibition reaction is launched between with HRP labeled Anti-IL6 autoantibody and unlabeled Anti-IL6 autoantibody with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Anti-IL6 autoantibody in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview : IL-6 is a m µLtifunctional protein produced by lymphoid and non-lymphoid cells, and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vasc µLar endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these various cells is reg µLated, either positively or negatively, by a variety of signals including mitogens, antigenic stim µLation, lipopolysaccharides, IL-1 TNF, PDGF and viruses. On the basis of its various activities, IL-6 has also been called interferon-?2 (IFN-?2), 26 kDa protein, B-cell stim µLatory factor-2 (BSF-2),?hybridoma/plasmacytoma growth factor, hepatocyte stim µLating factor, cytotoxic T-cell differentiation factor, and macrophage-gran µLocyte inducing factor 2A (MGI-2A).
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).