Volume : 50 µg
Clone Number :
Aliases : Egln3Egl nine homolog 3 antibody; EC 1.14.11.29 antibody; Hypoxia-inducible factor prolyl hydroxylase 3 antibody; HIF-PH3 antibody; HIF-prolyl hydroxylase 3 antibody; HPH-3 antibody; Prolyl hydroxylase domain-containing protein 3 antibody; PHD3 antibody; SM-20 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Mus musc µLus (Mouse)
UniProt ID : Q91UZ4
Immunogen : Recombinant Mouse Egl nine homolog 3 protein (2-239AA)
Raised in : Rabbit
Species Reactivity : Mouse
Tested Applications : ELISA
Background : Cell µLar oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation res µLting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. ELGN3 is the most important isozyme in limiting physiological activation of HIFs (partic µLarly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and reg µLates the stability of ADRB2. Reg µLator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably thro µgh reg µLating CASP3 activity. Also essential for hypoxic reg µLation of neutrophilic inflammation.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : Egln3
Research Areas : Epigenetics and Nuclear Signaling; Cancer; Cardiovasc µLar; Metabolism