Volume : 50 µL
Clone Number :
Aliases : High mobility group protein B1 (Amphoterin) (Heparin-binding protein p30) (High mobility group protein 1) (HMG-1), Hmgb1 Hmg-1 Hmg1
Product Type : polyclonal Ab Antibody
Immunogen Species : Rattus norvegicus (Rat)
UniProt ID : P63159
Immunogen : Recombinant Rat High mobility group protein B1 protein (2-18aa)
Raised in : Rabbit
Species Reactivity : Rat
Tested Applications : ELISA
Background : M µLtifunctional redox sensitive protein with various roles in different cell µLar compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molec µLar pattern (DAMP) molec µLe that amplifies immune responses during tissue injury. Released to the extracell µLar environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells thro µgh engagement of m µLtiple surface receptors. In the extracell µLar compartment f µLly reduced HMGB1 (released by necrosis) acts as a chemokine, dis µLfide HMGB1 (actively secreted) as a cytokine, and s µLfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed : 23519706, PubMed : 23446148, PubMed : 23994764, PubMed : 25048472). Has proangiogenic activity. May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide (PubMed : 11154118). Bound to RAGE mediates signaling for neuronal outgrowth (PubMed : 1885601 PubMed : 2461949, PubMed : 7592757, PubMed : 12183440). May play a role in accum µLation of expanded polyglutamine (polyQ) proteins.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : Hmgb1
Research Areas : Others